Assay Method Information | |
| ChEMBL_2035562 (CHEMBL4689720) |
Description: | Inhibition of N-terminal hexa-His-tagged human SIRT1 N346A mutant (156 to 664 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation equilibrium constant using Abz-GVLKacAYNO2GY-NH2 as substrate in presence of varying level of NAD+ by Lineweaver-burk plot analysis |
Affinity data for this assay | |
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