| Assay Method Information | |
| | ChEMBL_1562430 (CHEMBL3778166) |
| Description: | Inhibition of full length His-MBP-att-EGLN3 (1 to 239 residues) (unknown origin) in Escherichia coli BL21(DE3)pRR692 cells assessed as hydroxylation of the cy-5 labelled C-terminal oxygen dependent domain of substrate Hif2A preincubated for 30 mins followed by substrate addition measured after 40 mins by TR-FRET assay |
| Affinity data for this assay | |
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