Assay Method Information

Assay Name:  ChEMBL_2484872
Description:  Inhibition of N-terminal Strep tagged/TEC fused human IDH1 R132H mutant extracted from Escherichia coli BL21 (DE3) cells using alpha-ketoglutarate as substrate assessed as reduction in 2-HG levels preincubated for 30 mins in presence of NADPH followed by substrate addition by LC-MS/MS analysis
Affinity data for this assay
 

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