| Assay Method Information | |
| | ChEMBL_2035565 (CHEMBL4689723) |
| Description: | Inhibition of N-terminal hexa-His-tagged human SIRT1 F414A mutant (156 to 664 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation equilibrium constant using Abz-GVLKacAYNO2GY-NH2 as substrate in presence of varying level of NAD+ by Lineweaver-burk plot analysis |
| Affinity data for this assay | |
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