Report error Found 210 Enz. Inhib. hit(s) with Target = 'Bromodomain-containing protein 4' and Ligand = 'BDBM50365262'
Affinity DataIC50: 3.90nMAssay Description:Inhibition of BRD4 BD2 (unknown origin) preincubated for 15 mins followed by peptide addition and measured after 60 mins by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 5.20nMAssay Description:Binding affinity to human partial length BRD4 BD2 (K333 to E460 residues) expressed in bacterial expression system using H4K5acIC8acK12acK16ac-biotin...More data for this Ligand-Target Pair
Affinity DataKi: 5.30nMAssay Description:Binding affinity to human BRD4 BD1 (44 to 168 residues) by fluorescence polarization assayMore data for this Ligand-Target Pair
Affinity DataIC50: 5.30nMAssay Description:Inhibition of C-terminal His6-tagged BRD4 BD2 (unknown origin) using H-YSGRGK(Ac)GGK(Ac)GLGK(Ac)-GGAK(Ac)RHRK-Biotin-OH as substrate incubated for 1 ...More data for this Ligand-Target Pair
Affinity DataIC50: 5.70nMAssay Description:Inhibition of BRD4 BD2 (unknown origin) incubated for 15 mins by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataKd: 6.70nMAssay Description:Binding affinity to biotinylated BRD4 BD2 (333 to 460 amino acid residues) (unknown origin) expressed in Rosetta2 DE3 cells by bio-layer interferomet...More data for this Ligand-Target Pair
Affinity DataKi: 7.60nMAssay Description:Displacement of FAM-labeled ZBA248 from BRD4 BD1 (44 to 168 amino acid residues) (unknown origin) expressed in Rosetta2 DE3 cells after 30 mins by fl...More data for this Ligand-Target Pair
Affinity DataIC50: 7.90nMAssay Description:Inhibition of BRD4 BD2 (unknown origin) by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 8nMAssay Description:Inhibition of BRD4 bromodomain-1 (unknown origin) by europium based LANCE TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataKd: 8.20nMAssay Description:Binding affinity to recombinant human N-terminal hexaHis-tagged BRD4 expressed in Escherichia coli BL21 (DE3) cells by qPCR assayMore data for this Ligand-Target Pair
Affinity DataKd: 9nMAssay Description:Binding affinity to BRD4 BD1 (unknown origin) by thermal denaturation assayMore data for this Ligand-Target Pair
Affinity DataIC50: 9nMAssay Description:Displacement of biotinylated acetylated histone H4 peptide from human recombinant N-terminal GST-tagged BRD4 BD1 (49 to 170 residues) expressed in Es...More data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of BRD4 BD1 (unknown origin) incubated for 15 mins by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataKi: 11nMAssay Description:Displacement of FAM-labeled ZBA248 from BRD4 BD2 (333 to 460 amino acid residues) (unknown origin) expressed in Rosetta2 DE3 cells after 30 mins by f...More data for this Ligand-Target Pair
Affinity DataKi: 12nMAssay Description:Inhibition of FAM-labeled ZBA248 binding to recombinant human N-terminal His6-tagged BRD4 bromodomain 2 (333 to 460 residues) expressed in Rosetta2 D...More data for this Ligand-Target Pair
Affinity DataKi: 12nMAssay Description:Inhibition of FAM-labeled ZBA248 binding to recombinant N-terminal His6-tagged BRD4 bromodomain 2 (333 to 460 residues) (unknown origin) expressed in...More data for this Ligand-Target Pair
Affinity DataIC50: 12nMAssay Description:Inhibition of BRD4 BD1 (unknown origin) preincubated for 15 mins followed by peptide addition and measured after 60 mins by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:Inhibition of BRD4 BD2 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataKd: 13nMAssay Description:Binding affinity to human BRD4 BD1 (N44 to E168 aa residues) by bromoKdELECT Discover assayMore data for this Ligand-Target Pair
Affinity DataKd: 13nMAssay Description:Binding affinity to biotinylated BRD4 BD1 (44 to 168 amino acid residues) (unknown origin) expressed in Rosetta2 DE3 cells by bio-layer interferometr...More data for this Ligand-Target Pair
Affinity DataIC50: 14nMpH: 7.4 T: 2°CAssay Description:BRD4(2): Assays were performed as described previously with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents wer...More data for this Ligand-Target Pair
Affinity DataKd: 14nMAssay Description:Binding affinity to BRD4 BD2 (unknown origin) by thermal denaturation assayMore data for this Ligand-Target Pair
Affinity DataIC50: 14nMAssay Description:Inhibition of C-terminal His6-tagged BRD4 BD1 (unknown origin) using H-YSGRGK(Ac)GGK(Ac)GLGK(Ac)-GGAK(Ac)RHRK-Biotin-OH as substrate incubated for 1 ...More data for this Ligand-Target Pair
Affinity DataIC50: 15nMAssay Description:Inhibition of BRD4-BD1 (unknown origin) assessed as peptide-biotin titration - BRD4(1)/histone H4 peptide interaction by HTRF/EPIgeneous assayMore data for this Ligand-Target Pair
Affinity DataIC50: 15nMAssay Description:Inhibition of BRD4 BD2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataKi: 15nMAssay Description:Inhibition of FAM-labeled ZBA248 binding to recombinant human N-terminal His6-tagged BRD4 bromodomain 1 (44 to 168 residues) expressed in Rosetta2 DE...More data for this Ligand-Target Pair
Affinity DataKi: 15nMAssay Description:Inhibition of FAM-labeled ZBA248 binding to recombinant N-terminal His6-tagged BRD4 bromodomain 1 (44 to 168 residues) (unknown origin) expressed in ...More data for this Ligand-Target Pair
Affinity DataIC50: 17nMAssay Description:Inhibition of BRD4 BD2 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of BRD4 BD1 (unknown origin) by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMpH: 7.4 T: 2°CAssay Description:BRD4(1): Assays were performed as described previously with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents wer...More data for this Ligand-Target Pair
Affinity DataKd: 19nMAssay Description:Binding affinity to BRD4 D1 (unknown origin) assessed as dissociation constant by isothermal titration calorimetryMore data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of human BRD4 BD2 (342 to 460 residues) at 30 uM preincubated for 30 mins followed by substrate addition measured after 30 mins by TR-FRET...More data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Inhibition of human recombinant His-tagged BRD4 BD1 domain using H-SGRGK(Ac)GGK(Ac)GLGK-(Ac)GGAK(Ac)RHRK(Biotin)-OH as substrate preincubated with en...More data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Inhibition of BRD4 bromodomain 1 (unknown origin) by Alphascreen based methodMore data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Binding affinity to human BRD4 BD1 (44 to 168 residues) by fluorescence polarization assayMore data for this Ligand-Target Pair
Affinity DataIC50: 23nMAssay Description:Inhibition of BRD4 BD1 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 24nMAssay Description:Displacement of biotinylated tetra-acetylated histone H4 from human his-tagged BRD4 bromodomain1 by alphascreen assayMore data for this Ligand-Target Pair
Affinity DataIC50: 24nMAssay Description:Inhibition of His/FLAG-tagged BRD4 BD1 (unknown origin) using histone H4-TetraAcetylated-biotin peptide as substrate incubated for 20 mins by AlphaLI...More data for this Ligand-Target Pair
Affinity DataIC50: 24nMAssay Description:Inhibition of His-FLAG-tagged BRD4 binding domain1 (unknown origin) binding to H4-TetraAc-biotin peptide after 20 mins by AlphaLISAMore data for this Ligand-Target Pair
Affinity DataIC50: 25nMAssay Description:Displacement of tetra-acetylated histone H4 peptide (1-21) from recombinant human N-terminal His-tagged BRD4 BD2 (349 to 460 residues) expressed in E...More data for this Ligand-Target Pair
Affinity DataIC50: 27nMAssay Description:Expression and purification of the BRD4(I) recognition domain: colonies of newly transformed plasmid DNA from E. coli BL21(DE3)-condon plus-RIL cells...More data for this Ligand-Target Pair
Affinity DataKd: 27nMAssay Description:Binding affinity towards BRD4-BD1 (unknown origin) measured by isothermal titration calorimetry (ITC) methodMore data for this Ligand-Target Pair
Affinity DataIC50: 28nMAssay Description:Inhibition of recombinant human GST-tagged BRD4 BD1 (44 to 168 residues) expressed in Escherichia coli incubated for 60 mins by alphascreen assayMore data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Displacement of 5-FITC labelled (+)-JQ1 from His6-tagged human BRD4 bromodomain 1 expressed in Escherichia coli BL21(DE3) )-codon plus-RIL cells incu...More data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Displacement of FITC-JQ1 from His6-tagged BRD4 bromodomain-1 (unknown origin) expressed in Escherichia coli BL21(DE3) after 4 hrs by fluorescence ani...More data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Inhibition of BRD4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Inhibition of recombinant BRD4-BD1 (unknown origin) by TR-FRET assayMore data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Displacement of 5-FITC labelled (+)-JQ1 from His6-tagged human BRD4 bromodomain 1 expressed in Escherichia coli BL21(DE3) )-codon plus-RIL cells incu...More data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Inhibition of human N-terminal 6His-tagged BRD4 BD2 (351 to 457 residues) expressed in Escherichia coli BL21-CodonPlus (DE3) cells using biotinylated...More data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:ssays were performed as described previously with minor modifications from the manufacturer's protocol (PerkinElmer, USA). All reagents were dilu...More data for this Ligand-Target Pair
