Found 58 Enz. Inhib. hit(s) with Target = 'Dihydrofolate reductase' and Ligand = 'BDBM66082' 
Affinity DataKi: 0nMAssay Description:Inhibitory activity against dihydrofolate reductase (DHFR) of L-1210 cellsMore data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataKi: 0.0100nMAssay Description:Inhibition of DHFR (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 0nMAssay Description:Concentration required to inhibit Lactobacillus casei derived Dihydrofolate reductase activity by 50%More data for this Ligand-Target Pair
Affinity DataIC50: 2nMAssay Description:Inhibition of human DHFR assessed as reduction in consumption of NADPH using DHF as substrate preincubated for 1 min followed by DHF addition and mea...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of human DHFRMore data for this Ligand-Target Pair
Affinity DataIC50: 4.70nMAssay Description:Inhibition of human DHFR expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed by substrate and...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMAssay Description:Inhibition of recombinant human DHFR measured by spectrophotometric methodMore data for this Ligand-Target Pair
Affinity DataIC50: 6nMAssay Description:Inhibition of DHFR in human KB cells expressing RFC/FRalpha/PCFT assessed as reduction in cell growth after 96 hrs by Cell-Titer Blue assayMore data for this Ligand-Target Pair
Affinity DataIC50: 7.10nMAssay Description:Inhibition of recombinant human DHFR assessed as reduction in consumption of NADPH using DHF as substrate measured every 30 secs over 6 mins by spect...More data for this Ligand-Target Pair
Affinity DataIC50: 7.90nMAssay Description:Inhibition of recombinant human DHFR assessed as reduction in conversion of DHF to THF measured every 30 secs for 6 mins by UV-Vis spectrophotometric...More data for this Ligand-Target Pair
Affinity DataIC50: 8.80nMAssay Description:Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli BL21(DE3) assessed as reduction in consumption of NADPH using DHF a...More data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of DHFR in human KB cells expressing RFC/FRalpha/PCFT assessed as reduction in cell growth after 96 hrs in presence of folic acid by Cell-...More data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Inhibition of rat hippocampal Gamma-aminobutyric acid type B receptor (weak agonist)More data for this Ligand-Target Pair
Affinity DataIC50: 25nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolic acid as substrate in presence of NADPH after 15 mins by microplate reader analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolate as substrate after 15 mins in presence of NADPHMore data for this Ligand-Target Pair
Affinity DataIC50: 35nMAssay Description:Inhibition of Mycobacterium tuberculosis His-tagged DHFR assessed as reduction in consumption of NADPH using DHF as substrate measured every 30 secs ...More data for this Ligand-Target Pair
Affinity DataIC50: 35nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolate as substrate after 15 mins in presence of NADPHMore data for this Ligand-Target Pair
Affinity DataIC50: 61nMAssay Description:Inhibition of recombinant human DHFR expressed in Escherichia coli incubated for 15 mins and measured by ELISAMore data for this Ligand-Target Pair
Affinity DataIC50: 83nMAssay Description:Inhibition of recombinant human DHFR using dihydrofolate as substrate in presence of NADPHMore data for this Ligand-Target Pair
Affinity DataIC50: 88nMAssay Description:Inhibition of DHFR (unknown origin) incubated for 2 to 5 mins by absorbance based analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 1.00E+3nMAssay Description:Inhibition of human dihydrofolate reductase using dihydrofolic acid as substrate preincubated for 1 hr in presence of NADPH followed by substrate add...More data for this Ligand-Target Pair
Affinity DataIC50: 2.17E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by free MTX in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 2.42E+4nMAssay Description:Concentration required for inhibition of beef liver dihydrofolate reductaseMore data for this Ligand-Target Pair
Affinity DataIC50: 2.64E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by free MTX in experiment 1More data for this Ligand-Target Pair
Affinity DataIC50: 7.92E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and IgG1 in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 7.92E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
Affinity DataIC50: 8.80E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
Affinity DataIC50: 8.80E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and Dal K-20 in experime...More data for this Ligand-Target Pair
Affinity DataIC50: 8.80E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (7 M/M of IgG)and Dal K-20 in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 9.24E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and IgG1 in experiment 1More data for this Ligand-Target Pair
Affinity DataIC50: 9.24E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (4 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
Affinity DataIC50: 9.46E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 9.90E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (7 M/M of IgG)and Dal K-20 in experiment 1More data for this Ligand-Target Pair
Affinity DataIC50: 9.90E+4nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and Dal K-20 in experime...More data for this Ligand-Target Pair
Affinity DataIC50: 1.01E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate (regiospecific coupling) of MTX (6 M/M of IgG)and rabbit IgG (NRG) in expe...More data for this Ligand-Target Pair
Affinity DataIC50: 1.07E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (6 M/M of IgG)and IgG1 in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 1.10E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (5M/M of IgG) and rabbit IgG (NRG) in ...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (7 M/M of IgG) and rabbit IgG (NRG) in...More data for this Ligand-Target Pair
Affinity DataIC50: 1.14E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 1.16E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 1More data for this Ligand-Target Pair
Affinity DataIC50: 1.25E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (6 M/M of IgG) and rabbit IgG (NRG) in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 1.27E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate of MTX (6 M/M of IgG) and rabbit IgG (NRG) in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 1.27E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (4 M/M of IgG) and rabbit IgG (NRG) in experiment 2More data for this Ligand-Target Pair
Affinity DataIC50: 1.29E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by hydrazone-linked conjugate (regiospecific coupling) of MTX (5M/M of IgG) and rabbit IgG (NRG) in ...More data for this Ligand-Target Pair
Affinity DataIC50: 1.32E+5nMAssay Description:Inhibition of beef liver dihydrofolate reductase by amide-linked conjugate of MTX (7 M/M of IgG)and Dal K-20 in experiment 1More data for this Ligand-Target Pair
