Affinity DataIC50: 0.100nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMAssay Description:Inhibition of BLK (unknown origin) incubated for 1 hr in presence of ATP by Z'LYTE assayMore data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMAssay Description:Inhibition of recombinant full length human His-tagged BLK cytoplasmic domain expressed in baculovirus expression system using tyrosine-1 peptide as ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.200nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 uM ATP.). Reaction condition...More data for this Ligand-Target Pair
Affinity DataIC50: 0.200nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 μM ATP.). For enzyme in...More data for this Ligand-Target Pair
Affinity DataIC50: 0.200nMAssay Description:Inhibition of recombinant human BLK using Poly(Glu, Tyr) 4:1 as substrate after 1 hr by ELISAMore data for this Ligand-Target Pair
Affinity DataIC50: 0.230nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 0.230nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Inhibition of BLK (unknown origin) using peptide substrate in presence of ATP by caliper electrophoresis methodMore data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Inhibition of recombinant human BLK using Poly(Glu, Tyr) 4:1 as substrate after 1 hr by ELISAMore data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 μM ATP.). For enzyme in...More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 uM ATP.). Reaction condition...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: <0.508nMAssay Description:Table 3 and 4: The HotSpot kinase profiling and screening assays were carried out using the method of Anastassiadis et al., Nat. Biotechnol. (2011) V...More data for this Ligand-Target Pair
Affinity DataIC50: 0.580nMAssay Description:Preparation of Compounds to be Tested:1) Using DMSO to prepare 50× compound stock solutions (same as the stock solution in Example 34) for later use;...More data for this Ligand-Target Pair
Affinity DataIC50: 0.700nMAssay Description:Inhibition of BLK (unknown origin) using STK as substrate by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr](4:1) as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr] (4:1) as substrate preincubated for 60 mins followed by [gamma-33P]-ATP addition and measured after 120 m...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of human BLK using poly[Glu:Tyr](4:1) as substrate by [gamma-33P]-ATP assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of BLK (unknown origin) by ADP-Glo assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.40nMAssay Description:Inhibition of BLK (unknown origin) by Z'LYTE assayMore data for this Ligand-Target Pair
Ligand Info
Affinity DataIC50: 1.60nMAssay Description:Preparation of Compounds to be Tested:1) Using DMSO to prepare 50× compound stock solutions (same as the stock solution in Example 34) for later use;...More data for this Ligand-Target Pair
Affinity DataIC50: 2.20nMAssay Description:Inhibition of BLK (unknown origin) using peptide substrate in presence of ATP by caliper electrophoresis methodMore data for this Ligand-Target Pair
Affinity DataIC50: 2.5nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 2.70nMAssay Description:Inhibition of Blk (unknown origin) after 1 hr by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.20nMAssay Description:Inhibition of Blk (unknown origin) after 1 hr by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: <5nMAssay Description:IC50 Table 13-20: The protocol calls for test compound of the invention to be incubated with kinase, substrate, cofactors, and radio-isotope-labeled ...More data for this Ligand-Target Pair
Affinity DataIC50: <5nMAssay Description:IC50 Table 13-20: The protocol calls for test compound of the invention to be incubated with kinase, substrate, cofactors, and radio-isotope-labeled ...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 5.40nMAssay Description:Preparation of Compounds to be Tested:1) Using DMSO to prepare 50× compound stock solutions (same as the stock solution in Example 34) for later use;...More data for this Ligand-Target Pair
Affinity DataIC50: 5.5nMAssay Description:Inhibition of (S)-3-(3-(5-acrylamido-6-(3-(5-(2-methyl-5-(3-(trifluoromethyl)benzamido)benzamido)pyrimidin-2-ylamino)phenylamino)-6-oxohexylamino)-3-...More data for this Ligand-Target Pair
Affinity DataIC50: 5.60nMT: 2°CAssay Description:In vitro enzyme activity assay: wild-type and various mutants (T790M, L858R, L861Q, L858 R/T790M) EGFR, Z′-Lyte Kinase Assay Kit were purchased...More data for this Ligand-Target Pair
Affinity DataIC50: 5.90nMAssay Description:Inhibition of wild type N-terminal GST tagged BLK (unknown origin) (1 to 505 residues) expressed in sf21 insect cell using TK as substrate incubated ...More data for this Ligand-Target Pair
Affinity DataIC50: 6.10nMAssay Description:Inhibition of wild type N-terminal GST tagged BLK (unknown origin) (1 to 505 residues) expressed in sf21 insect cell using TK as substrate incubated ...More data for this Ligand-Target Pair
Affinity DataIC50: 6.30nMAssay Description:Inhibition of BLK (unknown origin) using peptide substrate in presence of ATPMore data for this Ligand-Target Pair
TargetTyrosine-protein kinase Blk(Mus musculus)
Center For Molecular Medicine Of The Austrian Academy Of Sciences
Curated by ChEMBL
Center For Molecular Medicine Of The Austrian Academy Of Sciences
Curated by ChEMBL
Affinity DataIC50: 7.30nMAssay Description:Inhibition of mouse BLKMore data for this Ligand-Target Pair
Affinity DataIC50: 7.40nMAssay Description:The compound of Example 39, 2-(1H-indol-5-ylamino)-6-(2,4-difluorophenylsulfonyl)-8-methylpyrido[2,3-d]pyrimidin-7(8H)-one, was subjected to a kinase...More data for this Ligand-Target Pair
Affinity DataIC50: 8nMAssay Description:Inhibition of recombinant full length His-tagged human Blk cytoplasmic domain expressed in Baculovirus expression system by Z-LYTE assayMore data for this Ligand-Target Pair
TargetTyrosine-protein kinase Blk(Mus musculus)
Center For Molecular Medicine Of The Austrian Academy Of Sciences
Curated by ChEMBL
Center For Molecular Medicine Of The Austrian Academy Of Sciences
Curated by ChEMBL
Affinity DataIC50: 8nMAssay Description:Inhibition of mouse BLKMore data for this Ligand-Target Pair
TargetTyrosine-protein kinase Blk(Mus musculus)
Center For Molecular Medicine Of The Austrian Academy Of Sciences
Curated by ChEMBL
Center For Molecular Medicine Of The Austrian Academy Of Sciences
Curated by ChEMBL
Affinity DataIC50: 8nMAssay Description:Inhibition of mouse BLK using ATP as substrateMore data for this Ligand-Target Pair
Affinity DataIC50: 8.10nMAssay Description:Inhibition of full-length recombinant human His-tagged BLK expressed in baculovirus by Z'-LYTE assayMore data for this Ligand-Target Pair
Affinity DataIC50: 8.30nMAssay Description:Inhibition of recombinant full length human His-tagged BLK cytoplasmic domain expressed in baculovirus expression system using tyrosine-1 peptide as ...More data for this Ligand-Target Pair
Affinity DataIC50: 8.30nMAssay Description:Inhibition of BLK (unknown origin)More data for this Ligand-Target Pair