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Target
Serine protease 1
Ligand
BDBM14325
Substrate
BDBM12679
Meas. Tech.
Enzyme Assay and Determination of the Inhibition Constants
pH
7.5±n/a
Temperature
295.15±n/a K
Ki
74±n/a nM
Comments
pH where Ki is lowest (pH(min)), and Ki(min) toward bovine trypsin.
Citation
 Katz, BA; Elrod, K; Verner, E; Mackman, RL; Luong, C; Shrader, WD; Sendzik, M; Spencer, JR; Sprengeler, PA; Kolesnikov, A; Tai, VW; Hui, HC; Breitenbucher, JG; Allen, D; Janc, JW Elaborate manifold of short hydrogen bond arrays mediating binding of active site-directed serine protease inhibitors. J Mol Biol 329:93-120 (2003) [PubMed]  Article 
Target
Name:
Serine protease 1
Synonyms:
Beta-Trypsin | Cationic trypsin | PRSS1 | TRP1 | TRY1 | TRY1_BOVIN | TRYP1 | Trypsin | Trypsin I
Type:
Enzyme
Mol. Mass.:
25790.52
Organism:
Bos taurus (bovine)
Description:
P00760
Residue:
246
Sequence:
MKTFIFLALLGAAVAFPVDDDDKIVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRVASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQKNKPGVYTKVCNYVSWIKQTIASN
  
Inhibitor
Name:
BDBM14325
Synonyms:
2-{5-[amino(iminiumyl)methyl]-1H-1,3-benzodiazol-2-yl}-6-phenylbenzen-1-olate | CRA-7806
Type:
Small organic molecule
Emp. Form.:
C20H16N4O
Mol. Mass.:
328.3672
SMILES:
NC(=[NH2+])c1ccc2nc([nH]c2c1)-c1cccc(-c2ccccc2)c1[O-]
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM12679
Synonyms:
BDBM13790 | BDBM14298 | Chromogenic Substrate
Type:
Small organic molecule
Emp. Form.:
C26H34N6O7S
Mol. Mass.:
574.649
SMILES:
Cc1ccc(cc1)S(=O)(=O)NCC(=O)N1CCCC1C(=O)NC(CCCCN)C(=O)Nc1ccc(cc1)[N+]([O-])=O
Structure:
Search PDB for entries with ligand similarity: