Reaction Details Report a problem with these data
Target
Serine/threonine-protein kinase Chk1
Ligand
BDBM33206
Substrate
biotinylated CHKtide
Meas. Tech.
CHK1 AlphaScreen
pH
7.5±n/a
Temperature
296.15±n/a K
Comments
51.4 +/- 1.2 inhibition @ 250 uM.
Citation
 Matthews, TP; Klair, S; Burns, S; Boxall, K; Cherry, M; Fisher, M; Westwood, IM; Walton, MI; McHardy, T; Cheung, KM; Van Montfort, R; Williams, D; Aherne, GW; Garrett, MD; Reader, J; Collins, I Identification of inhibitors of checkpoint kinase 1 through template screening. J Med Chem 52:4810-9 (2009) [PubMed]  Article 
Target
Name:
Serine/threonine-protein kinase Chk1
Synonyms:
CHEK1 | CHK1 | CHK1 checkpoint homolog | CHK1_HUMAN | Checkpoint kinase-1 (CHK1)
Type:
Enzyme Catalytic Domain
Mol. Mass.:
54443.02
Organism:
Homo sapiens (Human)
Description:
gi_166295192
Residue:
476
Sequence:
MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT
  
Inhibitor
Name:
BDBM33206
Synonyms:
thiazoloazepinyl acetamide, 5
Type:
Small organic molecule
Emp. Form.:
C9H11N3O2S
Mol. Mass.:
225.268
SMILES:
CC(=O)Nc1nc2CCCNC(=O)c2s1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
biotinylated CHKtide
Synonyms:
n/a
Type:
biotinylated peptide
Mol. Mass.:
3148.69
Organism:
n/a
Description:
n/a
Residue:
27
Sequence:
ITINKKKVSRSGLYRSPSMPENLNRPR