Affinity DataKi: 0.0300nMAssay Description:Antagonistic activity against estrogen receptor alpha in presence of 0.1 nM estradiolMore data for this Ligand-Target Pair
Affinity DataKi: 0.0400nMAssay Description:Antagonistic activity against estrogen receptor alpha in presence of 0.1 nM estradiolMore data for this Ligand-Target Pair
Affinity DataKi: 0.0700nMAssay Description:Antagonist activity at full length recombinant human ERalpha expressed in baculovirus expression system assessed as inhibition of estradiol-induced E...More data for this Ligand-Target Pair
Affinity DataKi: 0.0910nMAssay Description:Displacement of [3H]estradiol from human recombinant ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.100nMAssay Description:In vitro inhibition of [6,7-3H]-17beta-estradiol binding to human estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.130nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.150nMAssay Description:Relative binding affinity for human estrogen receptor alpha compared to [3H]-estradiolMore data for this Ligand-Target Pair
Affinity DataKi: 0.160nMAssay Description:Displacement of [2,4,6,7-3H]estradiol from human ERalpha expressed in HeLa cells after 18 hrs by liquid scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.180nMAssay Description:Displacement of [3H]estradiol from human recombinant ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.180nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.200nMAssay Description:Displacement of [3H]estradiol from human recombinant ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.200nM ΔG°: -13.1kcal/mole EC50: 1.14nMpH: 7.5 T: 2°CAssay Description:The binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer. The Kd for 3H-estradiol was determin...More data for this Ligand-Target Pair
Affinity DataKi: 0.210nM ΔG°: -13.2kcal/molepH: 7.5 T: 2°CAssay Description:The method employed was adapted from the scientific literature and described in detail by Osbourn et al. (1993, Biochemistry, 32, 6229-6236). Recombi...More data for this Ligand-Target Pair
Affinity DataKi: 0.210nMpH: 7.5Assay Description:The method employed was adapted from the scientific literature and described in detail by Osbourn et al. (1993, Biochemistry, 32, 6229-6236). Recombi...More data for this Ligand-Target Pair
Affinity DataKi: 0.210nMAssay Description:Displacement of [3H]estradiol from human recombinant ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.210nM ΔG°: -13.2kcal/molepH: 7.5 T: 2°CAssay Description:The method employed was adapted from the scientific literature and described in detail by Osboum et al. (1993, Biochemistry, 32, 6229-6236). Recombin...More data for this Ligand-Target Pair
Affinity DataKi: 0.220nMAssay Description:Ability to displace [3H]-17-beta-estradiol from Estrogen receptor alpha by scintillation proximity assay.More data for this Ligand-Target Pair
Affinity DataKi: 0.230nM ΔG°: -13.1kcal/molepH: 7.5 T: 2°CAssay Description:The method employed was adapted from the scientific literature and described in detail by Osbourn et al. (1993, Biochemistry, 32, 6229-6236). Recombi...More data for this Ligand-Target Pair
Affinity DataKi: 0.230nMpH: 7.5Assay Description:The method employed was adapted from the scientific literature and described in detail by Osbourn et al. (1993, Biochemistry, 32, 6229-6236). Recombi...More data for this Ligand-Target Pair
Affinity DataKi: 0.230nM ΔG°: -13.1kcal/molepH: 7.5 T: 2°CAssay Description:The method employed was adapted from the scientific literature and described in detail by Osboum et al. (1993, Biochemistry, 32, 6229-6236). Recombin...More data for this Ligand-Target Pair
Affinity DataKi: 0.240nMAssay Description:Displacement of [3H]estradiol from human recombinant ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.240nMAssay Description:Displacement of [3H]estradiol from human recombinant ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.25nM ΔG°: -13.0kcal/mole EC50: 3.18nMpH: 7.5 T: 2°CAssay Description:The binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer. The Kd for 3H-estradiol was determin...More data for this Ligand-Target Pair
Affinity DataKi: 0.25nMAssay Description:Displacement of [3H]estradiol from ERalpha after 4 hrs by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.25nMAssay Description:Binding affinity to ERalpha ligand binding domainMore data for this Ligand-Target Pair
Affinity DataKi: 0.25nMAssay Description:In vitro displacement of 0.5 nM [3H]17-beta-estradiol from human Estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.270nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.280nMAssay Description:Binding affinity for Estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.300nMAssay Description:Displacement of [3H]17beta-estradiol from ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.300nMAssay Description:Displacement of E2-Alexa633 from GFP-tagged ERalpha expressed in COS7 cells by FACSMore data for this Ligand-Target Pair
Affinity DataKi: 0.310nMAssay Description:Run the competition binding assay in a buffer containing 50 mM HEPES, pH 7.5, 1.5 mM EDTA, 150 mM NaCl, 10% glycerol, 1 mg/mL ovalbumin, and 5 mM DTT...More data for this Ligand-Target Pair
Affinity DataKi: 0.310nMAssay Description:The purpose of the following ER competition binding assays is to determine the affinity of a test compound against ERα (wild type), ERα (Y5...More data for this Ligand-Target Pair
Affinity DataKi: 0.310nMAssay Description:The purpose of the following ER competition binding assays is to determine the affinity of a test compound against ERα (wild type), ERα (Y5...More data for this Ligand-Target Pair
Affinity DataKi: 0.320nMAssay Description:Displacement of [3H]estradiol from human recombinant ERalphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.320nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.340nMAssay Description:Run the competition binding assay in a buffer containing 50 mM HEPES, pH 7.5, 1.5 mM EDTA, 150 mM NaCl, 10% glycerol, 1 mg/mL ovalbumin, and 5 mM DTT...More data for this Ligand-Target Pair
Affinity DataKi: 0.340nMAssay Description:The purpose of the following ER competition binding assays is to determine the affinity of a test compound against ERα (wild type), ERα (Y5...More data for this Ligand-Target Pair
Affinity DataKi: 0.340nMAssay Description:The purpose of the following ER competition binding assays is to determine the affinity of a test compound against ERα (wild type), ERα (Y5...More data for this Ligand-Target Pair
Affinity DataKi: 0.350nM ΔG°: -12.8kcal/molepH: 7.5 T: 2°CAssay Description:The binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer. The Kd for 3H-estradiol was determin...More data for this Ligand-Target Pair
Affinity DataKi: 0.350nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.360nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.360nMAssay Description:Displacement of [3H]-estradiol from Estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataKi: 0.370nMAssay Description:Inhibition constant was evaluated against Histamine N-methyl-transferaseMore data for this Ligand-Target Pair
Affinity DataKi: 0.370nM ΔG°: -12.7kcal/mole EC50: 4.32nMpH: 7.5 T: 2°CAssay Description:The binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer. The Kd for 3H-estradiol was determin...More data for this Ligand-Target Pair
Affinity DataKi: 0.370nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.370nMAssay Description:Displacement of [3H]estradiol from full length recombinant human ESR1 expressed in insect cells by radiometric assayMore data for this Ligand-Target Pair
Affinity DataKi: 0.370nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.370nMAssay Description:Displacement of [3H]estradiol from ERalpha after 4 hrs by scintillation countingMore data for this Ligand-Target Pair
Affinity DataKi: 0.380nMAssay Description:Binding affinity to ERalpha ligand binding domainMore data for this Ligand-Target Pair
Affinity DataKi: 0.380nMAssay Description:Displacement of [3H]estradiol from full-length human ERalpha receptor by scintillation countingMore data for this Ligand-Target Pair